Coding

Part:BBa_K174000

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-09-26)

SspB proteolysis chaperone

SspB protein targets proteins tagged with modified degradation tag, ssrA, and deliver them to ClpXP for proteolysis. Modified ssrA tags are fused onto the 3’ end of a gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Haotong Xiong
  • Summary: Added information collated from existing scientific studies

Additionally, sspB stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpXP in a non-ssrA-mediated fashion.

[1]
  1. ↑ “SspB - Stringent Starvation Protein B - Escherichia Coli (Strain K12) - SspB Gene & Protein.” n.d. Www.uniprot.org. https://www.uniprot.org/uniprot/P0AFZ3.
[edit]
Categories
//cds
//function/degradation
Parameters
n/asspB degradation protein